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Definitely a good idea Leon. For example could perhaps screen nanobody libraries with recombinant proteins. Monoclonal would be better, but I don’t feel sufficiently expert anymore in this area that has changed so much. Martin is still doing some (we collaborate a bit on this) but doing it the old fashion way.

Jacques

 

Jacques ROBERT, Ph. D. He/him/his 

Professor & Vice Chair, Microbiology & Immunology
Professor, Environmental Medicine
Director of the Xenopus laevis Research Resource for Immunobiology 

Program Director of the BS/MS IMV graduate program

Undergraduate Track Coordinator and Advisor for the BMB Concentration, UPBM

University of Rochester, Medical Center
Box 672, Room 2-11124, MRBX
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Phone: office: (585) 275-1722; lab: (585) 275-5359
Email: [log in to unmask]

 

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Xenopus laevis Resource for Immunobiology:

 http://www.urmc.rochester.edu/microbiology-immunology/resources/xenopus-laevis/

 

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From: Global Ranavirus Consortium <[log in to unmask]> on behalf of "Grayfer, Leon" <[log in to unmask]>
Reply-To: Global Ranavirus Consortium <[log in to unmask]>
Date: Wednesday, December 9, 2020 at 2:07 PM
To: "[log in to unmask]" <[log in to unmask]>
Subject: [EXT] Re: Special issue in Viruses: Ranaviruses and Anti-ranaviral Immunity

 

Thank you for considering, Greg. Maybe I can call on you to review a manuscript or two?

 

You are by no means over the hill! If you are submitting proposals, you're in the game!

 

I agree that what we need now are some good Abs. I have been thinking about this more and more. Maybe the four of us could submit an NSF EDGE (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=505480) proposal to generate tools and reagents with which to study FV3 and amphibian (maybe also fish and/or reptile) responses to them? I am certainly in a position to generate prokaryotic and eukaryotic-produced proteins with which to immunize mice/rabbits/chickens. We could propose to outsource the immunizations or do it in-house. We could also propose to generate individual/key tagged FV3 proteins that could be used for pull-down assay and fluorescently labeled proteins that could be used for cell localization experiments, such as in A6 and FHM cells. I could probably generate some preliminary data on the protein front within a couple of months. Please let me know your thoughts.

 

I agree that there is still much to be learned about FV3 as a pathogen and as a model of pathogen-host(s) coevolution. I am of the mind that as long as the topic motivates you, you should continue to pursue it. If being 'over the hill' is measured by proposal declines, then I am probably too far on the other side of that hill to even see it.  

 

Best wishes,

 

Leon

 


Leon Grayfer

Assistant Professor

Department of Biological Sciences

George Washington University

Science and Engineering Hall 5695

202-994-8076

 

 

On Wed, Dec 9, 2020 at 10:26 AM Greg Chinchar <[log in to unmask]> wrote:

Leon, 

 

I think I will pass on the offer.  There are better and more up-to-date folks to ask.  I guess I am not over the hill yet....but am getting there.  

 

Take care...stay safe.  A NSF proposal I submitted a year ago with James Jancovich and Tom Waltzek (designed to explore the function of FV3 genes) was not favorably reviewed (based largely on the absence of antibodies needed for pull-down and cell localization experiments).  I think at 73 it is time to pass the torch to another group of virologists.  I still think there is much to be learned using FV3 as a model...but it will need to be someone else to do it.  

 

Greg 

 

On Tue, Dec 8, 2020 at 4:48 PM Grayfer, Leon <[log in to unmask]> wrote:

You could always submit a review.

 

I am sure that your perspectives on the recent works in the context of previous literature would be very appreciated by everyone in the community, Greg!

 

Best wishes,

 

Leon

 

 

Leon Grayfer

Assistant Professor

Department of Biological Sciences

George Washington University

Science and Engineering Hall 5695

202-994-8076

 

 

On Tue, Dec 8, 2020 at 5:16 PM Greg Chinchar <[log in to unmask]> wrote:

Thanks....but as a semi- (or maybe fully) retired guy, I have nothing new to add.  

 

Greg 

 

On Tue, Dec 8, 2020 at 10:49 AM Grayfer, Leon <[log in to unmask]> wrote:

[External Email]

Dear friends and colleagues in the GRC,

 

I hope that you and your families are doing well and staying safe!

 

Viruses has asked me to put together a special issue on "Ranaviruses and Anti-ranaviral Immunity". I thought that this might be a great opportunity to bring together some of the recent work within our community.

 

Please visit the website for more details and consider contributing a manuscript or a review to this special issue.

 

 

The deadline for manuscript submission is May 31, 2021. 

 

 

I wish you all the very best and hope to see you in person sooner than later!

 

 

Leon

 

Leon Grayfer

Assistant Professor

Department of Biological Sciences

George Washington University

Science and Engineering Hall 5695

202-994-8076