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Thank you for considering, Greg. Maybe I can call on you to review a manuscript or two? You are by no means over the hill! If you are submitting proposals, you're in the game! I agree that what we need now are some good Abs. I have been thinking about this more and more. Maybe the four of us could submit an NSF EDGE ( https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=505480) proposal to generate tools and reagents with which to study FV3 and amphibian (maybe also fish and/or reptile) responses to them? I am certainly in a position to generate prokaryotic and eukaryotic-produced proteins with which to immunize mice/rabbits/chickens. We could propose to outsource the immunizations or do it in-house. We could also propose to generate individual/key tagged FV3 proteins that could be used for pull-down assay and fluorescently labeled proteins that could be used for cell localization experiments, such as in A6 and FHM cells. I could probably generate some preliminary data on the protein front within a couple of months. Please let me know your thoughts. I agree that there is still much to be learned about FV3 as a pathogen and as a model of pathogen-host(s) coevolution. I am of the mind that as long as the topic motivates you, you should continue to pursue it. If being 'over the hill' is measured by proposal declines, then I am probably too far on the other side of that hill to even see it. Best wishes, Leon Leon Grayfer Assistant Professor Department of Biological Sciences George Washington University Science and Engineering Hall 5695 202-994-8076 On Wed, Dec 9, 2020 at 10:26 AM Greg Chinchar <[log in to unmask]> wrote: > Leon, > > I think I will pass on the offer. There are better and more up-to-date > folks to ask. I guess I am not over the hill yet....but am getting there. > > Take care...stay safe. A NSF proposal I submitted a year ago with James > Jancovich and Tom Waltzek (designed to explore the function of FV3 genes) > was not favorably reviewed (based largely on the absence of antibodies > needed for pull-down and cell localization experiments). I think at 73 it > is time to pass the torch to another group of virologists. I still think > there is much to be learned using FV3 as a model...but it will need to be > someone else to do it. > > Greg > > On Tue, Dec 8, 2020 at 4:48 PM Grayfer, Leon <[log in to unmask]> > wrote: > >> You could always submit a review. >> >> I am sure that your perspectives on the recent works in the context of >> previous literature would be very appreciated by everyone in the >> community, Greg! >> >> Best wishes, >> >> Leon >> >> >> Leon Grayfer >> Assistant Professor >> Department of Biological Sciences >> George Washington University >> Science and Engineering Hall 5695 >> 202-994-8076 >> >> >> On Tue, Dec 8, 2020 at 5:16 PM Greg Chinchar <[log in to unmask]> >> wrote: >> >>> Thanks....but as a semi- (or maybe fully) retired guy, I have nothing >>> new to add. >>> >>> Greg >>> >>> On Tue, Dec 8, 2020 at 10:49 AM Grayfer, Leon < >>> [log in to unmask]> wrote: >>> >>>> *[External Email]* >>>> Dear friends and colleagues in the GRC, >>>> >>>> I hope that you and your families are doing well and staying safe! >>>> >>>> *Viruses* has asked me to put together a special issue on "Ranaviruses >>>> and Anti-ranaviral Immunity". I thought that this might be a great >>>> opportunity to bring together some of the recent work within our community. >>>> >>>> Please visit the website for more details and consider contributing a >>>> manuscript or a review to this special issue. >>>> >>>> https://www.mdpi.com/journal/viruses/special_issues/Ranaviruse_Immune >>>> >>>> The deadline for manuscript submission is May 31, 2021. >>>> >>>> >>>> I wish you all the very best and hope to see you in person sooner than >>>> later! >>>> >>>> >>>> Leon >>>> >>>> Leon Grayfer >>>> Assistant Professor >>>> Department of Biological Sciences >>>> George Washington University >>>> Science and Engineering Hall 5695 >>>> 202-994-8076 >>>> >>>