Dear Members of the GRC Listserv, If you work on amphibians, please see the message below from Lola Brooks, a PhD Candidate in the UK who is studying the refinement and replacement of non-model amphibians in research. Very Best, Amanda ____________________________________________ As a PhD candidate at the Institute of Zoology and Royal Veterinary college (UK), I am working on an NC3R's funded project to assess how we can better the application of reduction, refinement and replacement to the non-model amphibians disease work that is currently undertaken. In order to refine our work, I am keen to understand how we can record pain and suffering in some of the amphibians we work with when investigating the effects of diseases caused by chytrid fungus and ranavirus. I have designed a survey to understand which indicators of pain and suffering are currently used by those who work with amphibians and gain the prospectus of anyone who catches, handles and even just observes them, in order to help assess how recordable and measurable these indicators and behavioural signs are. Given the huge diversity of amphibians I am keen to encourage participation from a global audience and a broad range of people who work, or have worked with amphibians. The survey is in English, but I have had it translated into Portuguese and Spanish and each participant only needs to complete it once. I would be very grateful if it could be shared with others who work with amphibians too. Your participation is very much appreciated and please feel free to contact me directly if you have any question [log in to unmask] https://www.surveymonkey.co.uk/r/AMPH1B14N_WELFARE https://www.surveymonkey.com/r/AMPH1B14N_WELFARE_Portuguese https://www.surveymonkey.co.uk/r/AMPH1B14N_WELFARE_Spanish Many thanks and kind regards, Lola Lola Brookes PhD Candidate Institute of Zoology/ Royal Veterinary College [log in to unmask] [log in to unmask] www.zsl.org/science/users/lola-brookes