Dear all, we don’t have those either. Sorry Sven PS. We have got mabs against different ranavirus isolates which are not discriminating those viruses in cell cultures. May you use IHC or iIFAT instead of ISH? This is much easier. Dr. habil. Sven M. Bergmann Dr. med. vet. Dr. habil. agri. sci. – Fischereiwissenschaften Fachtierarzt für Fische Certified Veterinary Specialist for Aquaculture and for Fisheries Science Institut für Infektionsmedizin Institute of Infectology ___________________________________________ Friedrich-Loeffler-Institut Bundesforschungsinstitut für Tiergesundheit Federal Research Institute for Animal Health Südufer 10 | 17493 Greifswald - Insel Riems Tel: +49 38351 7 1103 Labor und 71150 Büro | Fax: +49 38351 7 1226 http://www.fli.bund.de/ Von: Global Ranavirus Consortium [mailto:[log in to unmask]] Im Auftrag von Greg Chinchar Gesendet: Mittwoch, 4. Mai 2016 03:17 An: [log in to unmask] Betreff: Re: Request for ranavirus histo blocks we don't have those. Greg On Tue, May 3, 2016 at 10:59 AM, Steckler,Natalie K <[log in to unmask]<mailto:[log in to unmask]>> wrote: Dear GRC members, I am a PhD student in Dr. Tom Waltzek’s laboratory at the University of Florida. As part of my research, we are currently developing a ranavirus-specific in situ hybridization assay. In order to complete the validation process, we are in need of histo blocks of infected tissues for the following ranaviruses: Ambystoma tigrinum virus (ATV), European catfish virus (ECV), and Santee-Cooper ranavirus (SCRV) [aka Largemouth bass virus or LMBV]. Would anyone be willing to provide any of these samples for this purpose? If you wish, we would be happy to return the remaining block to you at the conclusion of this project. Thank you to those of you who have already generously donated samples for this purpose. Please feel free to contact myself (Natalie Steckler – [log in to unmask]<mailto:[log in to unmask]>) and Dr. Tom Waltzek ([log in to unmask]<mailto:[log in to unmask]>) with any communications regarding this project. Thank you in advance for your time and consideration! Regards, Natalie Steckler --------------- Natalie Steckler, DVM, MS PhD Candidate, Aquatic Animal Health Infectious Diseases and Pathology University of Florida College of Veterinary Medicine ><((((º>