Dear all
We have a new PhD student working on ranavirus in the Netherlands.
He would like to become a member of the consortium.
His name is Bernardo Saucedo ([log in to unmask])
All the best
Gr
Marja
Van: Global Ranavirus Consortium [mailto:[log in to unmask]] Namens Gray,
Matt
Verzonden: donderdag 9 januari 2014 03:53
Aan: [log in to unmask]
Onderwerp: Book on Ranaviruses and Diagnostic Testing
Dear Rolando and GRC:
We were recently approved to write an eBook for Springer on ranaviruses;
attached is the proposal that Greg and I submitted. One of the chapters of
this book by Allan Pessier and Deb Miller will discuss diagnostic tools and
provide guidance for gold standards. The target publication date is
November 2014, so hopefully we’ll have initial suggestions on standards for
ranavirus diagnostics soon.
All the Best---
Matt
______________________________________________________________
Matthew J. Gray, Ph.D.
Director, Global Ranavirus Consortium
<http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm>
Center for Wildlife Health
University of Tennessee
274 Ellington Plant Sciences Building
Knoxville, TN 37996-4563
865.974.2740 [ofc] <mailto:[log in to unmask]>
[log in to unmask]
865.974.4714 [fax]
<http://fwf.ag.utk.edu/personnel/mgray.htm>
http://fwf.ag.utk.edu/personnel/mgray.htm
For more information about Forestry, Wildlife and Fisheries at UTK,
Please visit <http://fwf.ag.utk.edu/> http://fwf.ag.utk.edu/ or call
865.974.7126
_____________________________________________________________
From: Rolando Mazzoni <[log in to unmask]>
Reply-To: Global Ranavirus Consortium <[log in to unmask]>
Date: Monday, January 6, 2014 11:22 AM
To: "[log in to unmask]" <[log in to unmask]>
Subject: Re: Please Post to the the GRC
Hi all and a happy new year !
As I have already mentioned to some colleagues, we are interested on
sequencing our Brazilian FV3 isolates, so Thomas' proposal sounds exellent.
Actually we have ethanol preserved samples, extracted DNA and of course
infected live tadpoles and frogs. We will make arrangements about which
samples will be more suitable and how to ship them.
Besides Next-Gen sequencing we also discussed about some kind of
collaborative research on getting a "Gold standard" for RV detection. We
have seen during presentations and posters, as well as at the discussion
group on “Diagnostic” that several methods are being used (qPCR-RTPCR,
conventional PCR). However, it looks like nobody is using OIE protocols. So
we have the possibility of performing an experiment to compare sensitivity
and specificity of available methods for different RV isolates and strains
to suggest OIE a new “Gold Standard” for all RVs or recommended methods to
be applied on specific strains/species.
Best regards for all
Rolando
On Sun, Jan 5, 2014 at 8:10 PM, Thomas Waltzek <[log in to unmask]> wrote:
Hi Matt/Amanda,
Happy New Year! Please post the attached message to the GRC. I've also
copied the message below. All the best and cheers - Tom
Hello Everyone and Happy New Year!
At the 2013 International Ranavirus Symposium several of us (Brunner,
Storfer, Pessier, Jancovich, Garner, Wellehan, and Waltzek) met to discuss
the genomic sequencing of ranavirus isolates. The outcome of our informal
discussion was that each of the individual parties was going to sequence
their own isolates for their respective projects and then we would reconvene
to combine the datasets into a final global analysis. Toward that end, a UF
PhD student (Sieara Claytor – many of you met her at the meeting) working
under Drs. Susan Cameron Devitt, James Wellehan, and myself has made great
progress toward sequencing North American FV3 isolates.
The objectives of her study are to investigate the phylogeographic structure
of FV3 across its range, determine any patterns among hosts and/or
geography, to determine the origin of introductions, and to evaluate
climatic correlates of FV3. She has surveyed phylogenetically informative
loci (31 loci including hypervariable and conserved regions) from FV3-like
isolates across diverse host and temporospatial scales (2 bull frogs, 4 wood
frogs, pig frog, Columbia spotted frog, tiger frog, leopard frog, common
midwife toad, pallid sturgeon, white sturgeon, soft-shell turtle, 2 box
turtles, Smoky Mountain salamander, 3 yellow-spotted salamanders, and
Chinese giant salamander). Sieara’s next step is sequence an additional 20
North American isolates we have grown up and purified for genomic sequencing
on our NextGen MiSeq machine (Russian sturgeon, fathead minnow, Northern
pike, walleye, green frog, 3 more box turtles, 2 gopher tortoises, sulcata
tortoise, etc).
We are asking for all those interested in collaborating to provide FV3 and
FV3-like isolates to extend the geographic and taxonomic scope of the
project. If anyone has material (e.g. isolates, infected tissues, or
infected tissue DNA) that you would be willing to share in exchange for
authorship with this project, please let me know and I can help arrange
shipping of the samples. A special thanks to all those that have already
provided input and isolates as collaborators on this project (Matt Gray,
Debra Miller, James Jancovich, Jesse Brunner, James Wellehan, April Johnson,
Matt Allender, and Ellen Ariel).
We are hoping to have all samples by February 31st in order to begin
sequencing the new material.
Sincerely,
Thomas B. Waltzek
[log in to unmask]
530-574-2976
--
Thomas B. Waltzek, MS, DVM, PhD
Assistant Professor
Infectious Diseases and Pathology
College of Veterinary Medicine
Bldg #1379, Mowry Road
Gainesville, FL 32610
Work: 352-273-5202
Cell: 530-574-2976
Fax: 352-392-4707
Email: [log in to unmask]
--
Rolando Mazzoni
DVM/PhD
Pesquisador de Pós-doutorado
CPA/EVZ/UFG
Tel: (62) 35211576
Cel: (62) 81786489
