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Many thanks and cheers - T On Thu, Jan 9, 2014 at 9:01 AM, Rijks, J.M. <[log in to unmask]> wrote: > Hi Matt, Nice initiative. > > > > Thomas and others, to our knowledge we have not detected FV3 isolates yet, > so we are not going to be of much help. > > > > Kind regards, > > Jolianne Rijks > > > > > > *From:* Global Ranavirus Consortium [mailto:[log in to unmask]] *On > Behalf Of *Gray, Matt > *Sent:* donderdag 9 januari 2014 3:53 > *To:* [log in to unmask] > *Subject:* Book on Ranaviruses and Diagnostic Testing > > > > Dear Rolando and GRC: > > > > We were recently approved to write an eBook for Springer on ranaviruses; > attached is the proposal that Greg and I submitted. One of the chapters of > this book by Allan Pessier and Deb Miller will discuss diagnostic tools and > provide guidance for gold standards. The target publication date is > November 2014, so hopefully we’ll have initial suggestions on standards for > ranavirus diagnostics soon. > > > > All the Best--- > > Matt > > ______________________________________________________________ > Matthew J. Gray, Ph.D. > > Director, Global Ranavirus Consortium<http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm> > Center for Wildlife Health > > University of Tennessee > 274 Ellington Plant Sciences Building > Knoxville, TN 37996-4563 > 865.974.2740 [ofc] [log in to unmask] > 865.974.4714 [fax] > http://fwf.ag.utk.edu/personnel/mgray.htm > > > For more information about Forestry, Wildlife and Fisheries at UTK, > Please visit http://fwf.ag.utk.edu/ or call 865.974.7126 > _____________________________________________________________ > > > > > > *From: *Rolando Mazzoni <[log in to unmask]> > *Reply-To: *Global Ranavirus Consortium <[log in to unmask]> > *Date: *Monday, January 6, 2014 11:22 AM > *To: *"[log in to unmask]" <[log in to unmask]> > *Subject: *Re: Please Post to the the GRC > > > > Hi all and a happy new year ! > > > > As I have already mentioned to some colleagues, we are interested on > sequencing our Brazilian FV3 isolates, so Thomas' proposal sounds exellent. > > Actually we have ethanol preserved samples, extracted DNA and of course > infected live tadpoles and frogs. We will make arrangements about which > samples will be more suitable and how to ship them. > > > > Besides Next-Gen sequencing we also discussed about some kind of > collaborative research on getting a "Gold standard" for RV detection. We > have seen during presentations and posters, as well as at the discussion > group on “Diagnostic” that several methods are being used (qPCR-RTPCR, > conventional PCR). However, it looks like nobody is using OIE protocols. So > we have the possibility of performing an experiment to compare sensitivity > and specificity of available methods for different RV isolates and strains > to suggest OIE a new “Gold Standard” for all RVs or recommended methods to > be applied on specific strains/species. > > > > Best regards for all > > > > Rolando > > > > > > On Sun, Jan 5, 2014 at 8:10 PM, Thomas Waltzek <[log in to unmask]> wrote: > > Hi Matt/Amanda, > > Happy New Year! Please post the attached message to the GRC. I've > also copied the message below. All the best and cheers - Tom > > > > > > Hello Everyone and Happy New Year! > > > > At the 2013 International Ranavirus Symposium several of us (Brunner, > Storfer, Pessier, Jancovich, Garner, Wellehan, and Waltzek) met to discuss > the genomic sequencing of ranavirus isolates. The outcome of our informal > discussion was that each of the individual parties was going to sequence > their own isolates for their respective projects and then we would > reconvene to combine the datasets into a final global analysis. Toward that > end, a UF PhD student (Sieara Claytor – many of you met her at the meeting) > working under Drs. Susan Cameron Devitt, James Wellehan, and myself has > made great progress toward sequencing North American FV3 isolates. > > > > The objectives of her study are to investigate the phylogeographic > structure of FV3 across its range, determine any patterns among hosts > and/or geography, to determine the origin of introductions, and to evaluate > climatic correlates of FV3. She has surveyed phylogenetically informative > loci (31 loci including hypervariable and conserved regions) from FV3-like > isolates across diverse host and temporospatial scales (2 bull frogs, 4 > wood frogs, pig frog, Columbia spotted frog, tiger frog, leopard frog, > common midwife toad, pallid sturgeon, white sturgeon, soft-shell turtle, 2 > box turtles, Smoky Mountain salamander, 3 yellow-spotted salamanders, and > Chinese giant salamander). Sieara’s next step is sequence an additional 20 > North American isolates we have grown up and purified for genomic > sequencing on our NextGen MiSeq machine (Russian sturgeon, fathead minnow, > Northern pike, walleye, green frog, 3 more box turtles, 2 gopher tortoises, > sulcata tortoise, etc). > > > > We are asking for all those interested in collaborating to provide FV3 and > FV3-like isolates to extend the geographic and taxonomic scope of the > project. If anyone has material (e.g. isolates, infected tissues, or > infected tissue DNA) that you would be willing to share in exchange for > authorship with this project, please let me know and I can help arrange > shipping of the samples. A special thanks to all those that have already > provided input and isolates as collaborators on this project (Matt Gray, > Debra Miller, James Jancovich, Jesse Brunner, James Wellehan, April > Johnson, Matt Allender, and Ellen Ariel). > > > > We are hoping to have all samples by February 31st in order to begin > sequencing the new material. > > > > > > Sincerely, > > Thomas B. Waltzek > > [log in to unmask] > > 530-574-2976 > > > -- > Thomas B. Waltzek, MS, DVM, PhD > Assistant Professor > Infectious Diseases and Pathology > College of Veterinary Medicine > Bldg #1379, Mowry Road > Gainesville, FL 32610 > Work: 352-273-5202 > Cell: 530-574-2976 > Fax: 352-392-4707 > Email: [log in to unmask] > > > > > > -- > Rolando Mazzoni > > DVM/PhD > > Pesquisador de Pós-doutorado > > CPA/EVZ/UFG > > Tel: (62) 35211576 > > Cel: (62) 81786489 > -- Thomas B. Waltzek, MS, DVM, PhD Assistant Professor Infectious Diseases and Pathology College of Veterinary Medicine Bldg #1379, Mowry Road Gainesville, FL 32610 Work: 352-273-5202 Cell: 530-574-2976 Fax: 352-392-4707 Email: [log in to unmask]