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Thanks David! I'll contact you soon. Cheers - Tom On Mon, Jan 6, 2014 at 12:17 PM, David Lesbarreres < [log in to unmask]> wrote: > same here, > we have extracted DNA, tissue samples and soon some sequences that we'll > be happy to share with the group. Mainly leopard and wood frogs but some > american toads and green frogs may be available. > Best wishes to all for 2014, wherever you are it's gotta be warmer than > here... > David > > > > "It takes all the running you can do to keep in the same place." > > Dr. David Lesbarrères, Associate Professor > Director, School of Graduate Studies > > Genetic & Ecology of Amphibians Research Group (GEARG) > Department of Biology - Laurentian University, http://gearg.jimdo.com/ > <http://gearg.jimdo.com/>Ramsey Lake Road, Sudbury, Ontario P3E 2C6, > Canada > phone: (705)675-1151 ext. 3232/2275 - Fax: (705)671-3840 > > >>> Rolando Mazzoni <[log in to unmask]> 06/01/14 12:09 >>> > Hi all and a happy new year ! > As I have already mentioned to some colleagues, we are interested on > sequencing our Brazilian FV3 isolates, so Thomas' proposal sounds exellent. > > Actually we have ethanol preserved samples, extracted DNA and of course > infected live tadpoles and frogs. We will make arrangements about which > samples will be more suitable and how to ship them. > > Besides Next-Gen sequencing we also discussed about some kind of > collaborative research on getting a "Gold standard" for RV detection. We > have seen during presentations and posters, as well as at the discussion > group on “Diagnostic” that several methods are being used (qPCR-RTPCR, > conventional PCR). However, it looks like nobody is using OIE protocols. So > we have the possibility of performing an experiment to compare sensitivity > and specificity of available methods for different RV isolates and strains > to suggest OIE a new “Gold Standard” for all RVs or recommended methods to > be applied on specific strains/species. > > Best regards for all > > Rolando > > > > > On Sun, Jan 5, 2014 at 8:10 PM, Thomas Waltzek <[log in to unmask]> wrote: > >> Hi Matt/Amanda, >> Happy New Year! Please post the attached message to the GRC. I've >> also copied the message below. All the best and cheers - Tom >> >> >> >> Hello Everyone and Happy New Year! >> >> >> >> At the 2013 International Ranavirus Symposium several of us (Brunner, >> Storfer, Pessier, Jancovich, Garner, Wellehan, and Waltzek) met to discuss >> the genomic sequencing of ranavirus isolates. The outcome of our informal >> discussion was that each of the individual parties was going to sequence >> their own isolates for their respective projects and then we would >> reconvene to combine the datasets into a final global analysis. Toward that >> end, a UF PhD student (Sieara Claytor – many of you met her at the meeting) >> working under Drs. Susan Cameron Devitt, James Wellehan, and myself has >> made great progress toward sequencing North American FV3 isolates. >> >> >> >> The objectives of her study are to investigate the phylogeographic >> structure of FV3 across its range, determine any patterns among hosts >> and/or geography, to determine the origin of introductions, and to evaluate >> climatic correlates of FV3. She has surveyed phylogenetically informative >> loci (31 loci including hypervariable and conserved regions) from FV3-like >> isolates across diverse host and temporospatial scales (2 bull frogs, 4 >> wood frogs, pig frog, Columbia spotted frog, tiger frog, leopard frog, >> common midwife toad, pallid sturgeon, white sturgeon, soft-shell turtle, 2 >> box turtles, Smoky Mountain salamander, 3 yellow-spotted salamanders, and >> Chinese giant salamander). Sieara’s next step is sequence an additional 20 >> North American isolates we have grown up and purified for genomic >> sequencing on our NextGen MiSeq machine (Russian sturgeon, fathead minnow, >> Northern pike, walleye, green frog, 3 more box turtles, 2 gopher tortoises, >> sulcata tortoise, etc). >> >> >> >> We are asking for all those interested in collaborating to provide FV3 >> and FV3-like isolates to extend the geographic and taxonomic scope of the >> project. If anyone has material (e.g. isolates, infected tissues, or >> infected tissue DNA) that you would be willing to share in exchange for >> authorship with this project, please let me know and I can help arrange >> shipping of the samples. A special thanks to all those that have already >> provided input and isolates as collaborators on this project (Matt Gray, >> Debra Miller, James Jancovich, Jesse Brunner, James Wellehan, April >> Johnson, Matt Allender, and Ellen Ariel). >> >> >> >> We are hoping to have all samples by February 31st in order to begin >> sequencing the new material. >> >> >> >> >> >> Sincerely, >> >> Thomas B. Waltzek >> >> [log in to unmask] >> 530-574-2976 >> >> >> >> -- >> Thomas B. Waltzek, MS, DVM, PhD >> Assistant Professor >> Infectious Diseases and Pathology >> College of Veterinary Medicine >> Bldg #1379, Mowry Road >> Gainesville, FL 32610 >> Work: 352-273-5202 >> Cell: 530-574-2976 >> Fax: 352-392-4707 >> Email: [log in to unmask] >> > > > > -- > Rolando Mazzoni > DVM/PhD > Pesquisador de Pós-doutorado > CPA/EVZ/UFG > Tel: (62) 35211576 > Cel: (62) 81786489 > -- Thomas B. Waltzek, MS, DVM, PhD Assistant Professor Infectious Diseases and Pathology College of Veterinary Medicine Bldg #1379, Mowry Road Gainesville, FL 32610 Work: 352-273-5202 Cell: 530-574-2976 Fax: 352-392-4707 Email: [log in to unmask]