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Dear GRC: A reminder to please send information about your laboratory or others that you use for diagnostics of ranavirus infection and disease to your continental representative by tomorrow,Friday, 30 August, 5:00 pm Eastern Daylight Time. Submission instructions are provided below this email. Continental Representatives: Please compile and forward laboratory information to me by Monday, 2 September, instead of Amanda. Congratulations are in order for Amanda --- she gave birth last night to a baby boy, Robert!! Other tasks the GRC is working on: (1) We are compiling suggestions for the GRC logo and will send a survey soon to vote for your preference, (2) We will be sending instructions by mid-September for submitting proposals to host the 2015 International Symposium on Ranaviruses, (3) Greg Chinchar and Ellen Ariel are working on the first draft of the bylaws for the GRC, and (4) I am uploading videos of everyone’s symposium presentations to an iTunes site, and will share them along with photos that Yumi Une’s husband and I took. If you do NOT what your video (or slides) posted, please let me know. Amanda has a partial list, but considering she is currently unavailable, please send an email to me by Monday, 2 September, and I will not post your information. If you approve posting, you do not need to send an email to me. As mentioned during the symposium, the GRC would like to update its website with interactive options. We received one lead from Darren Dunlap (USGS) of an individual that might be able to assist. If you have any leads of someone who has skills in website design, and would be willing to do it gratis or for a nominal fee, please let me know by 13 September. Lastly, the GRC Executive Board was curious if any individuals were planning to write summary papers over the symposium for submission to journals, newsletters, etc? Deb and I submitted a brief summary about the symposium and WDA conference for the newsletter of the Southeastern Section of the Wildlife Society. In 2011, we produced several publications; below are links. http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/Kerby2011.pdf http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/RobertandChinchar2011.pdf http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/Lesbarreresetal2011.pdf http://fwf.ag.utk.edu/mgray/ranavirus/2012Publications/MarschangandMiller2012.pdf It would be great if we could produce 1-2 similar publications. These deliverables will help us secure funding for the 2015 symposium. Thanks to everyone who attended and presented at the 2013 symposium. We received a lot of feedback, and look forward to making the 2015 symposium even better! All the Best, Matt Director, Global Ranavirus Consortium http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm ______________________________________________________________ Matthew J. Gray, Ph.D. Center for Wildlife Health University of Tennessee 274 Ellington Plant Sciences Building Knoxville, TN 37996-4563 865.974.2740 [ofc] [log in to unmask]<mailto:[log in to unmask]> 865.974.4714 [fax] http://fwf.ag.utk.edu/personnel/mgray.htm For more information about Forestry, Wildlife and Fisheries at UTK, Please visit http://fwf.ag.utk.edu/ or call 865.974.7126 _____________________________________________________________ From: <Gray>, Matt Gray <[log in to unmask]<mailto:[log in to unmask]>> Date: Monday, August 19, 2013 8:46 PM To: Global Ranavirus Consortium <[log in to unmask]<mailto:[log in to unmask]>> Subject: Re: Diagnostic laboratories All --- Please submit laboratory information to your continental representative (see below) in the format requested earlier (see instructions below). Continental representatives will compile laboratory information and send to Amanda for posting to the GRC website. Yumi Une, D.V.M., Ph.D. Asia Representative Email: [log in to unmask]<mailto:[log in to unmask]> Ellen Ariel, Ph.D. Australia Representative Email:[log in to unmask]<mailto:[log in to unmask]> PD Dr. vet. med. Rachel Marschang Europe Representative Email:[log in to unmask]<mailto:[log in to unmask]> Thomas B. Waltzek, D.V.M., Ph.D. North America Representative Email: [log in to unmask]<mailto:[log in to unmask]> Rolando Mazzoni, D.V.M., Ph.D. South America Representative Email: [log in to unmask]<mailto:[log in to unmask]> Thank you very much in advance for sharing this information! Best regards, Matt From: <Gray>, Matt Gray <[log in to unmask]<mailto:[log in to unmask]>> Date: Monday, August 19, 2013 7:19 AM To: Global Ranavirus Consortium <[log in to unmask]<mailto:[log in to unmask]>> Subject: RE: Diagnostic laboratories Dear Ann and GRC: There are a few resources with a partial list of diagnostic labs that test for ranavirus; see the following links: http://fwf.ag.utk.edu/mgray/SEPARC/SEPARCShippingFinal.pdf http://fwf.ag.utk.edu/mgray/Publications/Greenetal2009_DoddCh26.pdf (see page 494) Ann’s email highlights the need for a comprehensive list by continent of diagnostic laboratories, or research laboratories that are willing to process samples for individuals. To standardize the submission of information and reduce emails to the LISTSERV, please submit laboratory information to your GRC continental representative (see list at: http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm) by Friday, 30 August. Your representative will submit submissions to the GRC (Amanda Duffus), and we will post on the website for reference. Please format your submission as: LABORATORY NAME and CONTACT INFORMATION TYPE: Diagnostic, Research, or Both SERVICES OFFERED: (e.g., PCR, qPCR, ELISA, RFLP, MCP sequencing, genomic sequencing, virus culture, EM, histology, immunohistochemistry, etc) AVERAGE COST: Provide typical cost per sample for each test or a range; indicated currency For Example, University of Tennessee Center for Wildlife Health 274 Ellington Plant Sciences Building Knoxville TN 37996 USA 865-974-7126 Email: [log in to unmask]<mailto:[log in to unmask]> or [log in to unmask]<mailto:[log in to unmask]> http://wildlifehealth.tennessee.edu/ TYPE: Both SERVICES: PCR, qPCR, virus culture, MCP sequencing, histology Average Cost: $20 – $100 USD per sample depending on service(s) From: Andrew Storfer <[log in to unmask]<mailto:[log in to unmask]>> Reply-To: Global Ranavirus Consortium <[log in to unmask]<mailto:[log in to unmask]>> Date: Monday, August 19, 2013 8:14 PM To: "[log in to unmask]<mailto:[log in to unmask]>" <[log in to unmask]<mailto:[log in to unmask]>> Subject: Re: Diagnostic laboratories Hi, Our lab also performs real-time quantitative PCR testing for ranaviruses as well (using a MCP probe). For more information, please see the following website: http://sbs.wsu.edu/facilities/amphibian-disease-diagnostic-laboratory/ Best, Andrew On Mon, Aug 19, 2013 at 11:34 AM, Allan Pessier <[log in to unmask]<mailto:[log in to unmask]>> wrote: Our lab is offering real-time PCR for diagnostic or research applications on a fee-for-service and collaborative basis. We also can provide: 1) conventional PCR and sequencing (MCP, Pol and NF) for preliminary characterization of positive samples (e.g. FV3-like vs ATV-like); 2) PCR from ethanol or formalin-fixed paraffin embedded tissues (FFPE); and 3) Histopathology to support outbreak investigations or characterization of tissue changes associated with experimental infections. Details on submissions for qPCR can be found online at: http://www.sandiegozooglobal.org/News/Amphibian_Disease_Laboratory/ Cheers, Allan Allan P. Pessier, DVM, Dipl. ACVP Senior Scientist Wildlife Disease Laboratories Institute for Conservation Research San Diego Zoo Global P.O. Box 120551 San Diego, CA 92112-0551 (619) 231-1515, Ext 4510#<tel:%28619%29%20231-1515%2C%20Ext%204510%23> [log in to unmask]<mailto:[log in to unmask]> >>> "Brunner, Jesse L" <[log in to unmask]<mailto:[log in to unmask]>> 8/18/2013 5:44 PM >>> Hi Ann, That is something that the GRC was planning on offering. Perhaps we can use this opportunity to push up our due-date. Would those offering diagnostic services email me a list of what you can offer and the terms. For instance, my lab can perform quantitative real time PCR and isolate and titer live ranaviruses, but we generally only do small numbers of samples or collaborate with others on larger projects; we are not a fee-for-service operation. I have a web page with instructions for submission here: http://public.wsu.edu/~jesse.brunner/samples.html I'm sure there are others and it would be very useful to put together a list. We will add a page with this information on the GRC website once we revise it. Jesse Jesse Brunner School of Biological Sciences Washington State University PO Box 644236 Pullman, WA 99164 USA [log in to unmask]<mailto:[log in to unmask]> 509-335-3702<tel:509-335-3702>