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All ---
Please submit laboratory information to your continental representative (see below) in the format requested earlier (see instructions below).
Continental representatives will compile laboratory information and send to Amanda for posting to the GRC website.
Yumi Une, D.V.M., Ph.D.
Asia Representative
Email:
une@azabu-u.ac.jp
Ellen Ariel, Ph.D.
Australia
Representative
PD Dr. vet. med. Rachel Marschang
Europe Representative
Thomas B. Waltzek, D.V.M., Ph.D.
North America Representative
Email:
[log in to unmask]
Rolando Mazzoni, D.V.M., Ph.D.
South America Representative
Thank you very much in advance for sharing this information!
Best regards,
Matt
Dear Ann and GRC:
There are a few resources with a partial list of diagnostic labs that test for ranavirus; see the following links:
http://fwf.ag.utk.edu/mgray/SEPARC/SEPARCShippingFinal.pdf
http://fwf.ag.utk.edu/mgray/Publications/Greenetal2009_DoddCh26.pdf (see page 494)
Ann’s email highlights the need for a comprehensive list by continent of diagnostic laboratories, or research laboratories that are willing to process samples for individuals. To standardize the submission of information and reduce emails to the LISTSERV,
please submit laboratory information to your GRC continental representative (see list at: http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm)
by Friday, 30 August. Your representative will submit submissions to the GRC (Amanda Duffus), and we will post on the website for reference.
Please format your submission as:
LABORATORY NAME and CONTACT INFORMATION
TYPE: Diagnostic, Research, or Both
SERVICES OFFERED: (e.g., PCR, qPCR, ELISA, RFLP, MCP sequencing, genomic sequencing, virus culture, EM, histology, immunohistochemistry, etc)
AVERAGE COST: Provide typical cost per sample for each test or a range; indicated currency
For Example,
University of Tennessee Center for Wildlife Health
274 Ellington Plant Sciences Building
Knoxville TN 37996 USA
865-974-7126
Email: [log in to unmask] or [log in to unmask]
TYPE: Both
SERVICES: PCR, qPCR, virus culture, MCP sequencing, histology
Average Cost: $20 – $100 USD per sample depending on service(s)
Our lab is offering real-time PCR for diagnostic or research applications on a fee-for-service and collaborative basis. We also can provide: 1) conventional PCR and sequencing (MCP, Pol and NF) for preliminary characterization of positive samples (e.g. FV3-like vs ATV-like); 2) PCR from ethanol or formalin-fixed paraffin embedded tissues (FFPE); and 3) Histopathology to support outbreak investigations or characterization of tissue changes associated with experimental infections.Details on submissions for qPCR can be found online at:Cheers,Allan
Allan P. Pessier, DVM, Dipl. ACVP>>> "Brunner, Jesse L" <[log in to unmask]> 8/18/2013 5:44 PM >>>
Senior Scientist
Wildlife Disease Laboratories
Institute for Conservation Research
San Diego Zoo Global
Hi Ann,
That is something that the GRC was planning on offering. Perhaps we can use this opportunity to push up our due-date.
Would those offering diagnostic services email me a list of what you can offer and the terms. For instance, my lab can perform quantitative real time PCR and isolate and titer live ranaviruses, but we generally only do small numbers of samples or collaborate with others on larger projects; we are not a fee-for-service operation. I have a web page with instructions for submission here: http://public.wsu.edu/~jesse.brunner/samples.html
I'm sure there are others and it would be very useful to put together a list. We will add a page with this information on the GRC website once we revise it.
Jesse
Jesse BrunnerSchool of Biological SciencesWashington State UniversityPO Box 644236Pullman, WA 99164 USA