All --- Please submit laboratory information to your continental representative (see below) in the format requested earlier (see instructions below). Continental representatives will compile laboratory information and send to Amanda for posting to the GRC website. Yumi Une, D.V.M., Ph.D. Asia Representative Email: [log in to unmask]<mailto:[log in to unmask]> Ellen Ariel, Ph.D. Australia Representative Email: [log in to unmask]<mailto:[log in to unmask]> PD Dr. vet. med. Rachel Marschang Europe Representative Email: [log in to unmask]<mailto:[log in to unmask]> Thomas B. Waltzek, D.V.M., Ph.D. North America Representative Email: [log in to unmask]<mailto:[log in to unmask]> Rolando Mazzoni, D.V.M., Ph.D. South America Representative Email: [log in to unmask]<mailto:[log in to unmask]> Thank you very much in advance for sharing this information! Best regards, Matt From: <Gray>, Matt Gray <[log in to unmask]<mailto:[log in to unmask]>> Date: Monday, August 19, 2013 7:19 AM To: Global Ranavirus Consortium <[log in to unmask]<mailto:[log in to unmask]>> Subject: RE: Diagnostic laboratories Dear Ann and GRC: There are a few resources with a partial list of diagnostic labs that test for ranavirus; see the following links: http://fwf.ag.utk.edu/mgray/SEPARC/SEPARCShippingFinal.pdf http://fwf.ag.utk.edu/mgray/Publications/Greenetal2009_DoddCh26.pdf (see page 494) Ann’s email highlights the need for a comprehensive list by continent of diagnostic laboratories, or research laboratories that are willing to process samples for individuals. To standardize the submission of information and reduce emails to the LISTSERV, please submit laboratory information to your GRC continental representative (see list at: http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm) by Friday, 30 August. Your representative will submit submissions to the GRC (Amanda Duffus), and we will post on the website for reference. Please format your submission as: LABORATORY NAME and CONTACT INFORMATION TYPE: Diagnostic, Research, or Both SERVICES OFFERED: (e.g., PCR, qPCR, ELISA, RFLP, MCP sequencing, genomic sequencing, virus culture, EM, histology, immunohistochemistry, etc) AVERAGE COST: Provide typical cost per sample for each test or a range; indicated currency For Example, University of Tennessee Center for Wildlife Health 274 Ellington Plant Sciences Building Knoxville TN 37996 USA 865-974-7126 Email: [log in to unmask]<mailto:[log in to unmask]> or [log in to unmask]<mailto:[log in to unmask]> http://wildlifehealth.tennessee.edu/ TYPE: Both SERVICES: PCR, qPCR, virus culture, MCP sequencing, histology Average Cost: $20 – $100 USD per sample depending on service(s) From: Andrew Storfer <[log in to unmask]<mailto:[log in to unmask]>> Reply-To: Global Ranavirus Consortium <[log in to unmask]<mailto:[log in to unmask]>> Date: Monday, August 19, 2013 8:14 PM To: "[log in to unmask]<mailto:[log in to unmask]>" <[log in to unmask]<mailto:[log in to unmask]>> Subject: Re: Diagnostic laboratories Hi, Our lab also performs real-time quantitative PCR testing for ranaviruses as well (using a MCP probe). For more information, please see the following website: http://sbs.wsu.edu/facilities/amphibian-disease-diagnostic-laboratory/ Best, Andrew On Mon, Aug 19, 2013 at 11:34 AM, Allan Pessier <[log in to unmask]<mailto:[log in to unmask]>> wrote: Our lab is offering real-time PCR for diagnostic or research applications on a fee-for-service and collaborative basis. We also can provide: 1) conventional PCR and sequencing (MCP, Pol and NF) for preliminary characterization of positive samples (e.g. FV3-like vs ATV-like); 2) PCR from ethanol or formalin-fixed paraffin embedded tissues (FFPE); and 3) Histopathology to support outbreak investigations or characterization of tissue changes associated with experimental infections. Details on submissions for qPCR can be found online at: http://www.sandiegozooglobal.org/News/Amphibian_Disease_Laboratory/ Cheers, Allan Allan P. Pessier, DVM, Dipl. ACVP Senior Scientist Wildlife Disease Laboratories Institute for Conservation Research San Diego Zoo Global P.O. Box 120551 San Diego, CA 92112-0551 (619) 231-1515, Ext 4510#<tel:%28619%29%20231-1515%2C%20Ext%204510%23> [log in to unmask]<mailto:[log in to unmask]> >>> "Brunner, Jesse L" <[log in to unmask]<mailto:[log in to unmask]>> 8/18/2013 5:44 PM >>> Hi Ann, That is something that the GRC was planning on offering. Perhaps we can use this opportunity to push up our due-date. Would those offering diagnostic services email me a list of what you can offer and the terms. For instance, my lab can perform quantitative real time PCR and isolate and titer live ranaviruses, but we generally only do small numbers of samples or collaborate with others on larger projects; we are not a fee-for-service operation. I have a web page with instructions for submission here: http://public.wsu.edu/~jesse.brunner/samples.html I'm sure there are others and it would be very useful to put together a list. We will add a page with this information on the GRC website once we revise it. Jesse Jesse Brunner School of Biological Sciences Washington State University PO Box 644236 Pullman, WA 99164 USA [log in to unmask]<mailto:[log in to unmask]> 509-335-3702<tel:509-335-3702>