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Lab. of Vet. Pathol. Azabu University TYPE: Diagnostic Detection of ranavirus infection 1. PCR The profile of 3 primer sets which were used in our survey. If two or more of these primer sets were positive, that sample was determined to be positive. FV3 FV3MCP4F 5'-GACTTGGCCACTTATGAC-3' 530bp FV3MCP5R 5'-GTCTCTGGAGAAGAAGAA-3' JP RanaJP556F 5'-GGTTCTTCCCCTCCCATTCTTCTT-3' 217bp RanaJP772R 5'-GGTCATGTAGACGTTGGCCTCGAC-3' M68Ｆ M68F 5'-GCACCACCTCTACTCTTATG-3' 230bp BIVMCP154 5'-CCATCGAGCCGTTCATGATG-3' Diagnosis of ranaviral disease. 1. Pathological examination 2. PCR 3. Epidemiological analysis In Japan, Rana viruses 4 or more has been confirmed. I want a consensus primers that can detect these viruses. TYPE: Research PCR, MCP sequencing, genomic sequencing, virus culture, pathological examination. Our Lab do not have anti ranavirus antibody. 2013/8/29 Gray, Matt <[log in to unmask]> > Dear GRC:**** > > ** ** > > A reminder to please send information about your laboratory or others that > you use for diagnostics of ranavirus infection and disease to your > continental representative by tomorrow,*Friday, 30 August, 5:00 pm *Eastern > Daylight Time. Submission instructions are provided below this email. ** > ** > > ** ** > > Continental Representatives:**** > > ** ** > > Please compile and forward laboratory information to me by *Monday, 2 > September,* instead of Amanda. > > > *Congratulations are in order for Amanda --- she gave birth last night > to a baby boy, Robert!! ***** > > ** ** > > Other tasks the GRC is working on:**** > > ** ** > > (1) We are compiling suggestions for the GRC logo and will send a > survey soon to vote for your preference,**** > > (2) We will be sending instructions by mid-September for submitting > proposals to host the 2015 International Symposium on Ranaviruses,**** > > (3) Greg Chinchar and Ellen Ariel are working on the first draft of the > bylaws for the GRC, and**** > > (4) I am uploading videos of everyone’s symposium presentations to an > iTunes site, and will share them along with photos that Yumi Une’s husband > and I took. If you do NOT what your video (or slides) posted, please let > me know. Amanda has a partial list, but considering she is currently > unavailable, please send an email to me by *Monday, 2 September,* and I > will not post your information. If you approve posting, you *do not*need to send an email to me. > **** > > ** ** > > As mentioned during the symposium, the GRC would like to update its > website with interactive options. We received one lead from Darren Dunlap > (USGS) of an individual that might be able to assist. If you have any > leads of someone who has skills in website design, and would be willing to > do it gratis or for a nominal fee, please let me know *by 13 September*. * > *** > > ** ** > > Lastly, the GRC Executive Board was curious if any individuals were > planning to write summary papers over the symposium for submission to > journals, newsletters, etc? Deb and I submitted a brief summary about the > symposium and WDA conference for the newsletter of the Southeastern Section > of the Wildlife Society. In 2011, we produced several publications; below > are links. > > > http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/Kerby2011.pdf > > > http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/RobertandChinchar2011.pdf > > > http://fwf.ag.utk.edu/mgray/ranavirus/2011Publications/Lesbarreresetal2011.pdf > > > http://fwf.ag.utk.edu/mgray/ranavirus/2012Publications/MarschangandMiller2012.pdf > > > It would be great if we could produce 1-2 similar publications. These > deliverables will help us secure funding for the 2015 symposium.**** > > ** ** > > Thanks to everyone who attended and presented at the 2013 symposium. We > received a lot of feedback, and look forward to making the 2015 symposium > even better!**** > > ** ** > > All the Best,**** > > Matt**** > > Director, Global Ranavirus Consortium **** > > http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm**** > > ______________________________________________________________ > Matthew J. Gray, Ph.D. > Center for Wildlife Health**** > > University of Tennessee > 274 Ellington Plant Sciences Building > Knoxville, TN 37996-4563 > 865.974.2740 [ofc] [log in to unmask] > 865.974.4714 [fax] > http://fwf.ag.utk.edu/personnel/mgray.htm**** > > > For more information about Forestry, Wildlife and Fisheries at UTK, > Please visit http://fwf.ag.utk.edu/ or call 865.974.7126 > _____________________________________________________________ > > **** > > ** ** > > From: <Gray>, Matt Gray <[log in to unmask]> > Date: Monday, August 19, 2013 8:46 PM > To: Global Ranavirus Consortium <[log in to unmask]> > Subject: Re: Diagnostic laboratories > > All ---**** > > ** ** > > Please submit laboratory information to your continental representative > (see below) in the format requested earlier (see instructions below). > Continental representatives will compile laboratory information and send > to Amanda for posting to the GRC website.**** > > * * > > Yumi Une, D.V.M., Ph.D. **** > > Asia Representative**** > > Email: * *[log in to unmask]**** > > ** ** > > Ellen Ariel, Ph.D. **** > > Australia Representative**** > > Email:**[log in to unmask]**** > > ** ** > > PD Dr. vet. med. Rachel Marschang **** > > Europe Representative **** > > Email:**[log in to unmask]**** > > ** ** > > Thomas B. Waltzek, D.V.M., Ph.D.**** > > North America Representative **** > > Email: * *[log in to unmask]**** > > ** ** > > Rolando Mazzoni, D.V.M., Ph.D.**** > > South America Representative**** > > Email: [log in to unmask]**** > > ** ** > > Thank you very much in advance for sharing this information! > > > Best regards,**** > > Matt > > > From: <Gray>, Matt Gray <[log in to unmask]> > Date: Monday, August 19, 2013 7:19 AM > To: Global Ranavirus Consortium <[log in to unmask]> > Subject: RE: Diagnostic laboratories > > Dear Ann and GRC:**** > > ** ** > > There are a few resources with a partial list of diagnostic labs that test > for ranavirus; see the following links:**** > > ** ** > > http://fwf.ag.utk.edu/mgray/SEPARC/SEPARCShippingFinal.pdf**** > > ** ** > > http://fwf.ag.utk.edu/mgray/Publications/Greenetal2009_DoddCh26.pdf (see > page 494)**** > > ** ** > > Ann’s email highlights the need for a comprehensive list by continent of > diagnostic laboratories, or research laboratories that are willing to > process samples for individuals. To standardize the submission of > information and reduce emails to the LISTSERV, please submit laboratory > information to your GRC continental representative (see list at: > http://fwf.ag.utk.edu/mgray/ranavirus/Ranavirus.htm) by *Friday, 30 August > *. Your representative will submit submissions to the GRC (Amanda > Duffus), and we will post on the website for reference. **** > > ** ** > > *Please format your submission as*:**** > > ** ** > > LABORATORY NAME and CONTACT INFORMATION **** > > TYPE: Diagnostic, Research, or Both**** > > SERVICES OFFERED: (e.g., PCR, qPCR, ELISA, RFLP, MCP sequencing, genomic > sequencing, virus culture, EM, histology, immunohistochemistry, etc)**** > > AVERAGE COST: Provide typical cost per sample for each test or a range; > indicated currency**** > > ** ** > > *For Example,* > > ** ** > > University of Tennessee Center for Wildlife Health**** > > 274 Ellington Plant Sciences Building**** > > Knoxville TN 37996 USA**** > > 865-974-7126**** > > Email: [log in to unmask] or [log in to unmask]**** > > **http://wildlifehealth.tennessee.edu/** > > > TYPE: Both**** > > SERVICES: PCR, qPCR, virus culture, MCP sequencing, histology**** > > Average Cost: $20 - $100 USD per sample depending on service(s)**** > > ** ** > > > > From: Andrew Storfer <[log in to unmask]> > Reply-To: Global Ranavirus Consortium <[log in to unmask]> > Date: Monday, August 19, 2013 8:14 PM > To: "[log in to unmask]" <[log in to unmask]> > Subject: Re: Diagnostic laboratories > > Hi, > > Our lab also performs real-time quantitative PCR testing for ranaviruses > as well (using a MCP probe). For more information, please see the > following website: > > http://sbs.wsu.edu/facilities/amphibian-disease-diagnostic-laboratory/ > > Best, > Andrew > > > On Mon, Aug 19, 2013 at 11:34 AM, Allan Pessier <[log in to unmask]>wrote: > >> Our lab is offering real-time PCR for diagnostic or research >> applications on a fee-for-service and collaborative basis. We also can >> provide: 1) conventional PCR and sequencing (MCP, Pol and NF) >> for preliminary characterization of positive samples (e.g. FV3-like vs >> ATV-like); 2) PCR from ethanol or formalin-fixed paraffin embedded tissues >> (FFPE); and 3) Histopathology to support outbreak investigations or >> characterization of tissue changes associated with experimental infections. >> >> Details on submissions for qPCR can be found online at: >> >> http://www.sandiegozooglobal.org/News/Amphibian_Disease_Laboratory/ >> >> Cheers, >> >> Allan >> >> >> >> >> >> Allan P. Pessier, DVM, Dipl. ACVP >> Senior Scientist >> Wildlife Disease Laboratories >> Institute for Conservation Research >> San Diego Zoo Global >> P.O. Box 120551 >> San Diego, CA 92112-0551 >> (619) 231-1515, Ext 4510# >> [log in to unmask] >> >>> "Brunner, Jesse L" <[log in to unmask]> 8/18/2013 5:44 PM >>> >> Hi Ann, >> >> That is something that the GRC was planning on offering. Perhaps we can >> use this opportunity to push up our due-date. >> >> Would those offering diagnostic services email me a list of what you >> can offer and the terms. For instance, my lab can perform quantitative real >> time PCR and isolate and titer live ranaviruses, but we generally only do >> small numbers of samples or collaborate with others on larger projects; we >> are not a fee-for-service operation. I have a web page with instructions >> for submission here: http://public.wsu.edu/~jesse.brunner/samples.html >> >> I'm sure there are others and it would be very useful to put together a >> list. We will add a page with this information on the GRC website once we >> revise it. >> >> Jesse >> >> Jesse Brunner >> School of Biological Sciences >> Washington State University >> PO Box 644236 >> Pullman, WA 99164 USA >> [log in to unmask] >> 509-335-3702 >> >> >> -- 宇根 有美 麻布大学獣医学部病理学研究室 252-5201 神奈川県相模原市中央区淵野辺1‐17-71 042-769-1628(FAX兼用） Yumi Une, D.V.M., Ph. D., Professor, Laboratory of Veterinary Pathology, School of Veterinary Medicine Azabu University 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan Phone & Fax: +81-42-769-1628